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f4 80 antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc f4 80 antibody
    Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites <t>(F4/80</t> immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.
    F4 80 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f4+80/pmc13019081-258-0-5
    Average 86 stars, based on 1 article reviews
    f4 80 antibody - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "A catalytically active and recyclable bioelastomer inspired by metalloenzymes"

    Article Title: A catalytically active and recyclable bioelastomer inspired by metalloenzymes

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.053

    Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites (F4/80 immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.
    Figure Legend Snippet: Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites (F4/80 immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.

    Techniques Used: Staining, Immunohistochemistry, Expressing

    Related Articles

    Incubation:

    Article Title: Posttranscriptional reprogramming controls MASLD progression through chronic ER stress adaptation
    Article Snippet: .. Sections were incubated overnight at 4°C with primary antibodies against CD3 (IS50330, Dako) and F4/80 (70076, Cell Signaling Technology). ..

    Article Title: Posttranscriptional reprogramming controls MASLD progression through chronic ER stress adaptation.
    Article Snippet: .. Sections were incubated overnight at 4°C with primary antibodies against CD3 (IS50330, Dako) and F4/80 (70076, Cell Signaling Technology). ..

    Article Title: Excessive pyroptosis mediates the exacerbation of pneumonia caused by low-lethality influenza virus and secondary MRSA co-infection.
    Article Snippet: Then the slides were incubated with autofluorescence quencher solution (Wuhan Baiqiandu Biotechnology Co., Ltd, B0008) for 30min AR TI CL E IN P RE SS and 3%H2O2 to inhibit endogenous peroxidase. .. Following 30 min blocking with 3% bovine serum albumin, slides were incubated at 4°C for 24 h with primary antibody [GSDMD Rabbit pAb (ABclonal, China, Cat#A18281, 1:100); F4/80 (CST, USA, Cat#70076, 1:200)], which completely covered the tissue sample. ..

    Immunofluorescence:

    Article Title: PHB2 ameliorates ferroptosis and aortic aneurysm/dissection through NEDD4L-dependent ubiquitination of NCOA4
    Article Snippet: CD31 MicroBeads (130-097-418, Miltenyi Biotec, Germany) were used to isolate endothelial cells, followed by CD11b MicroBeads (130-049-601, Miltenyi Biotec) for macrophages, and the remaining CD31 − CD11b − fraction was collected as vascular smooth muscle cells. .. The purity of each cell population was verified by immunofluorescence staining for α-smooth muscle actin (α-SMA; 1:200, A5228, Sigma-Aldrich) for VSMCs, F4/80 (1:200, 70076, Cell Signaling Technology, USA) for macrophages, and CD31 (1:200, ab28364, Abcam, UK) for endothelial cells. ..

    Staining:

    Article Title: PHB2 ameliorates ferroptosis and aortic aneurysm/dissection through NEDD4L-dependent ubiquitination of NCOA4
    Article Snippet: CD31 MicroBeads (130-097-418, Miltenyi Biotec, Germany) were used to isolate endothelial cells, followed by CD11b MicroBeads (130-049-601, Miltenyi Biotec) for macrophages, and the remaining CD31 − CD11b − fraction was collected as vascular smooth muscle cells. .. The purity of each cell population was verified by immunofluorescence staining for α-smooth muscle actin (α-SMA; 1:200, A5228, Sigma-Aldrich) for VSMCs, F4/80 (1:200, 70076, Cell Signaling Technology, USA) for macrophages, and CD31 (1:200, ab28364, Abcam, UK) for endothelial cells. ..

    other:

    Article Title: A Context-Aware Single-Cell Proteomics Analysis pipeline
    Article Snippet: Slides were then scanned using a Nanozoomer slide scanner (Hamamatsu Photonics) and viewed using NDP.view 2 software.

    Blocking Assay:

    Article Title: Excessive pyroptosis mediates the exacerbation of pneumonia caused by low-lethality influenza virus and secondary MRSA co-infection.
    Article Snippet: Then the slides were incubated with autofluorescence quencher solution (Wuhan Baiqiandu Biotechnology Co., Ltd, B0008) for 30min AR TI CL E IN P RE SS and 3%H2O2 to inhibit endogenous peroxidase. .. Following 30 min blocking with 3% bovine serum albumin, slides were incubated at 4°C for 24 h with primary antibody [GSDMD Rabbit pAb (ABclonal, China, Cat#A18281, 1:100); F4/80 (CST, USA, Cat#70076, 1:200)], which completely covered the tissue sample. ..



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    Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites <t>(F4/80</t> immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.
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    Image Search Results


    Prolonged inflammatory cell accumulation in Ext1‐deficient mice during lipopolysaccharide (LPS)‐induced lung injury. (A) Immunohistochemical staining for CD11b + cells at 0–48 h after LPS administration. (B) Quantification of CD11b + cells. Ext1‐deficient mice (red line) show higher CD11b + cell retention at 48 h, indicating delayed inflammatory resolution. (C) Immunohistochemical staining for F4/80 + macrophages. (D) Quantification of F4/80 + cells. Cre + Ext1 flox/flox mice show persistent and increased macrophage accumulation, consistent with impaired inflammatory clearance. Data represent mean ± standard error of the mean (SEM). * p < 0.05 vs. Cre − controls.

    Journal: The FASEB Journal

    Article Title: Endothelial Exostosin‐1 Is Needed for Glycocalyx Integrity and Inflammatory Resolution in Acute Lung Injury

    doi: 10.1096/fj.202601250R

    Figure Lengend Snippet: Prolonged inflammatory cell accumulation in Ext1‐deficient mice during lipopolysaccharide (LPS)‐induced lung injury. (A) Immunohistochemical staining for CD11b + cells at 0–48 h after LPS administration. (B) Quantification of CD11b + cells. Ext1‐deficient mice (red line) show higher CD11b + cell retention at 48 h, indicating delayed inflammatory resolution. (C) Immunohistochemical staining for F4/80 + macrophages. (D) Quantification of F4/80 + cells. Cre + Ext1 flox/flox mice show persistent and increased macrophage accumulation, consistent with impaired inflammatory clearance. Data represent mean ± standard error of the mean (SEM). * p < 0.05 vs. Cre − controls.

    Article Snippet: Sections were incubated with primary antibodies against CD11b (Abcam, ab16667; 1:200), F4/80 (Bioss, bs‐10701R; 1:200), or heparan sulfate (10E4 clone; Amsbio, #370255; 1:100) overnight at 4°C.

    Techniques: Immunohistochemical staining, Staining

    YQHXF promoted M2 macrophage polarization in mice. ( A and B) Representative immunofluorescence images of macrophage distribution. (C) Immunofluorescence co-staining for detection the number of F4/80 + CD86 + positive cells (M1 macrophages). (D) Immunofluorescence co-staining for detection the number of F4/80 + CD206 + positive cells (M2 macrophages). Scale bar = 20 μm. Values are expressed as mean ± SD ( n = 3). Data were analyzed using one-way ANOVA. * p < 0.05, *** p < 0.001, compared with the control group; ## p < 0.01, ### p < 0.001, compared with the model group. YQHXF, Yiqihuoxue formula.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Yiqihuoxue formula improves atherosclerosis by regulating macrophage polarization associated with enhanced STAT6/PPARγ pathway

    doi: 10.3389/fcvm.2026.1896890

    Figure Lengend Snippet: YQHXF promoted M2 macrophage polarization in mice. ( A and B) Representative immunofluorescence images of macrophage distribution. (C) Immunofluorescence co-staining for detection the number of F4/80 + CD86 + positive cells (M1 macrophages). (D) Immunofluorescence co-staining for detection the number of F4/80 + CD206 + positive cells (M2 macrophages). Scale bar = 20 μm. Values are expressed as mean ± SD ( n = 3). Data were analyzed using one-way ANOVA. * p < 0.05, *** p < 0.001, compared with the control group; ## p < 0.01, ### p < 0.001, compared with the model group. YQHXF, Yiqihuoxue formula.

    Article Snippet: Antibodies information: anti-CD86, ER1906-01, 1:200, HUABIO; anti-CD206, ab64693, 1:200, Abcam; F4/80, 28463-1-AP, 1:400, Proteintech; anti-p-STAT6, HA722776, 1:200, HUABIO; anti-PPARγ, 16643-1-ap, 1:200, Proteintech; FITC labeled goat anti-rabbit, GB22303, 1:100, Servicebio; HRP labeled goat anti-rabbit, GB23303, 1:100, Servicebio.

    Techniques: Immunofluorescence, Staining, Control

    YQHXF promoted M2 macrophage polarization in HFD-treated mice is associated with STAT6/PPARγ signaling pathway. (A) Representative picture of STAT6, p-STAT6, and PPARγ proteins expression detected by Western blot; (B–D) Quantification of STAT6 (B) , p-STAT6 (C) , and PPARγ (D) proteins relative expression. β-actin was used to normalize the data. (E,F) Representative immunofluorescence images of F4/80 + p-STAT6 + (E) and F4/80 + PPARγ + (F) cells distribution. ( G,H) Quantitative analysis of F4/80 + p-STAT6 + (G) and F4/80 + PPARγ + (H) positive cells. Scale bar = 20 μm. Values are expressed as mean ± SD ( n = 3). Data were analyzed using one-way ANOVA. * p < 0.05, ** p < 0.05, compared with the control group; ## p < 0.01, ### p < 0.001, compared with the model group. HFD: high-fat diet; YQHXF: Yiqihuoxue formula.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Yiqihuoxue formula improves atherosclerosis by regulating macrophage polarization associated with enhanced STAT6/PPARγ pathway

    doi: 10.3389/fcvm.2026.1896890

    Figure Lengend Snippet: YQHXF promoted M2 macrophage polarization in HFD-treated mice is associated with STAT6/PPARγ signaling pathway. (A) Representative picture of STAT6, p-STAT6, and PPARγ proteins expression detected by Western blot; (B–D) Quantification of STAT6 (B) , p-STAT6 (C) , and PPARγ (D) proteins relative expression. β-actin was used to normalize the data. (E,F) Representative immunofluorescence images of F4/80 + p-STAT6 + (E) and F4/80 + PPARγ + (F) cells distribution. ( G,H) Quantitative analysis of F4/80 + p-STAT6 + (G) and F4/80 + PPARγ + (H) positive cells. Scale bar = 20 μm. Values are expressed as mean ± SD ( n = 3). Data were analyzed using one-way ANOVA. * p < 0.05, ** p < 0.05, compared with the control group; ## p < 0.01, ### p < 0.001, compared with the model group. HFD: high-fat diet; YQHXF: Yiqihuoxue formula.

    Article Snippet: Antibodies information: anti-CD86, ER1906-01, 1:200, HUABIO; anti-CD206, ab64693, 1:200, Abcam; F4/80, 28463-1-AP, 1:400, Proteintech; anti-p-STAT6, HA722776, 1:200, HUABIO; anti-PPARγ, 16643-1-ap, 1:200, Proteintech; FITC labeled goat anti-rabbit, GB22303, 1:100, Servicebio; HRP labeled goat anti-rabbit, GB23303, 1:100, Servicebio.

    Techniques: Expressing, Western Blot, Immunofluorescence, Analysis, Control

    Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites (F4/80 immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.

    Journal: Bioactive Materials

    Article Title: A catalytically active and recyclable bioelastomer inspired by metalloenzymes

    doi: 10.1016/j.bioactmat.2026.02.053

    Figure Lengend Snippet: Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites (F4/80 immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.

    Article Snippet: F4/80 antibody was purchased from Cell Signaling Technology (Danvers MA, Cat #: 70076).

    Techniques: Staining, Immunohistochemistry, Expressing